My lab is interested in understanding mechanisms responsible for changes in synaptic connectivity in the nervous system during development and disease. Our basic approach is to image nerve cells from transgenic mice expressing variants of green fluorescent protein (GFP) in the neuronal cytoplasm using confocal and fluorescent microscopy. We target these same nerve cells for a much more highly resolved serial electron microscopic reconstruction so that we can directly attribute subcellular alterations revealed by electron microscopy to more macroscopic observations made by light microscopy.  Three current projects in the lab include: developmental synapse elimination, axon regeneration/reinnervation, and spinal cord injury.